Electrophoresis Study for DNA Fragmentation in a Microfluidic Chip

Adrienne Hafley*, Peng Li, Ph. D, Xiaojun Li, M.S.
C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV 26506

Presentation Category: Physical Sciences & Engineering (Poster presentation)

Student’s Major: Chemistry

Biological testing efficiency has become increasingly important as researchers begin to address the need of quicker and simpler testing methods. Bulky and expensive machinery limits the reach of the laboratory, but smaller, more cost-effective testing devices can reach a wider population (creating an emphasis in point-of-care diagnostics). However, no method is effective if the results obtained are unclear or invalid. My research this semester is focused on working with Dr. Peng Li's research group in their endeavor to fragment DNA through a sharp-tip vibrating capillary. To validate the effectiveness of the fragmentation, gel electrophoresis is used to measure fragment size. By learning the different elements of gel electrophoresis and through experimentation, we were able to optimize the gel electrophoresis to give clear measurements that can test the Li group’s method of fragmentation. By studying various analytical techniques the research group uses, I am able to learn about the functions of the lab and the process the group uses to enhance and create biological testing technology.

Funding: Not funded

Program/mechanism supporting research/creative efforts: WVU's Research Apprenticeship Program (RAP) & accompanying HONR 297-level course